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KMID : 0903520090520050545
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2009 Volume.52 No. 5 p.545 ~ p.554
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A Subchronic Toxicity Study and in vitro Genotoxicity Studies of Conjugated Linoleic Acid-diglyceride Type Structured Lipid
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Hong Soon-Gi
Park Chae-Kyu
Cho Byung-Goo
Wee Jae-Joon
Hyun Sun-Hee
Chung Shin-Kyo
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Abstract
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In the present study, we synthesized a diglyceride (CLA-DG) from glycerol and conjugated linoleic acid (CLA). CLA-DG contained about 66% CLA as fatty acids. The safety of CLA-DG was assessed in acute and subchronic studies and in genotoxicity assays. In a single-dose acute study of CLA-DG, male and female rats were administered 5000 mg/kg body weight. CLA-DG caused no adverse effects and body weight gain and food consumption were within the normal range, thus the LD50 of CLA-DG was determined to be greater than 5000 mg/kg. In a 90-day subchronic study (500, 1000, and 2000 mg/kg b.w./day, oral via gavage) in male and female rats, no significant adverse effects in food consumption, body weight, mortality, clinical chemistry, hematology, gross pathology, and histopathology were observed. CLA-DG did not show any potential to induce gene mutations in reverse mutation tests using Salmonella typhimurium TA98, TA100, TA1535, TA1537, and Escherichia coli WP2uvrA strains. CLA-DG did not induce chromosomal aberrations in cultured Chinese hamster lung cells. The results from these studies support the safety of CLA-DG.
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KEYWORD
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chromosomal aberrations, conjugated linoleic acid, diglyceride, genotoxicity, reverse
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